Glucosidase II(beta) (K117) pAb

Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains (alpha) and (beta) subunits (Glucosidase II(alpha) and Glucosidase II(beta)). The (alpha) and (beta) subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase II(alpha) is the catalyitc core of the enzyme and can function independently of the (beta) subunit. The sequence of Glucosidase II(beta) encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase II(beta) is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase II(beta) was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase II(beta) retains the complete complex in the ER.

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SPECIFICATIONS

Catalog Number

BS2646

Size

100ug/100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 90-130 of Human Glucosidase II(beta).

Species

Glucosidase II(beta) (K117) pAb detects endogenous levels of Glucosidase II(beta) protein.

Dilutions

WB: 1:500~1:1000\nIF: 1:50~1:200

Swiss Prot

P14314

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul