GLUT1 (Glucose Transporter 1, Solute Carrier Family 2, Facilitated Glucose Transporter Member 1, Glucose Transporter Type 1, Erythrocyte/Brain, GLUT-1, HepG2 Glucose Transporter, SLC2A1)

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.\n\nApplications:\nSuitable for use in Western Blot and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-5ug/ml using chemiluminescence techniques. It is recommended not to heat the samples prior to loading to prevent gluts aggregation.\nFlow Cytometry: ~10ug/ml (10e6 cells); Perform the antibody binding in small volume of ~200ul.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman liver tumor cells (HepG2)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3900-01M

Size

100ug

Applications

FC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.5.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography

Isotype

IgG2b

References

1. Haspel, et al., JBC 263: 398 (1986). 2. Birnbaum, et al., 83: 5784-5788 (1986). 3. Piper et al., Am. J. Physiol. 260: C570 (1991). 4. Harris, et al., PNAS 89: 7556 (1992). 5. Baldwin, S.A., BBA 1154: 17 (1993). 6. Mueckler, M., Eur. J. Biochem. 219: 713 (1994).

Additional Info

Recognizes human GLUT1.