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Glycogen Synthase Kinase 3 beta (GSK3b)

Cat no: G8170-50R

Glycogen Synthase Kinase 3 beta (GSK3b)

Glycogen synthase kinase 3 (GSK-3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK-3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK-3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK-3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK-3 are ubiquitously expressed, although particularly high levels of GSK-3beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK-3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK-3a and Ser9 of GSK-3b, S6K, RSK, PKA and PKC), dephosphorylation (PP1 and PP2A), and by binding to protein complexes (with beta-catenin, axin, CK1 and the APC complex).\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000\nWestern Blot: 1:500-1:2000\nImmunohistochemistry (Paraffin): 1: 4000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8170-50R

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as liquid, 0.04% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG2

References

1. EMBO J. 1998 Mar 2;17(5):1371-84, 2. Curr Biol. 2001 Jan 9;11(1):44-9, 3. Cancer Lett. 2003 Sep 25;199(2):201-8.

Additional Info

Recognizes human GSK3B at 146kD. Species Crossreactivity: rat, monkey.

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