Granulocytes are nucleated white blood cells that have been classified according to the staining behavior of their cytoplasmic granules as neutrophils, eosinophils, or basophils. They are derived from the myeloid precursor series in the bone marrow and have a life span of only a few hours in peripheral blood. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. Eosinophils normally comprises about 3%, basophils comprise about 0.5% and neutrophils comprise about 60-80% of circulating WBCs. Eosinophils have large eosinophilic cytoplasmic granules and are prominent in parasitic infections and allergic reactions. Basophils have large basophilic granules that obscure the nucleus and are involved in type I hypersensitivity reactions. Neutrophils have a multilobate nucleus (for this reason they are also called polymorphonuclear leukocytes) and fine cytoplasmic granules. They participate in the nonspecific acute inflammatory response to injury. They are particularly active against extracellularly multiplying infectious agents, notably bacteria, but are also involved in repair and immune responses.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Positive Control:
Tonsil
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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