Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: ~10,000-15,000
Western Blot: 1:1000-1:2000
Immunohistochemistry: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Storage and Stability:
May be stored at 4 degrees C for short-term only. Do Not Freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.