Heat Shock Cognate Protein 70 (HSC70, Constitutive Heat Shock Protein 70, Heat Shock 70kD Protein 10, Heat Shock 70kD Protein 8, Heat Shock 70kDa Protein 8, Heat Shock Cognate 71kD Protein, Heat Shock Cognate Protein 54, HSC54, Heat Shock Cognate Protein

Hsp73 is a normal cell constituent which is synthesized in increased abundance under adverse environmental circumstances, such as non-physiological temperatures or treatment with amino acid analogues (1). Data has shown a molecular mechanism for cytokine-mediated posttranscriptional regulation of Bim mRNA by Hsc70, which binds to AU-rich elements (AREs) in the 3'-untranslated region of specific mRNAs and enhances their stability. The RNA binding potential of Hsc70 is regulated by cochaperones including Bag-4 (also SODD), CHIP, Hip, and Hsp40 (2). Hsp73 protein expression was detected in the cytoplasm of melanoma cells in primary tumors and metastases. In primary melanomas, the proportion of Hsp73 protein expressing cells correlated with tumor thickness which was found to be statistically significant.\n\nApplications:\nSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:30\nWestern Blot: 1:500\nImmunoprecipitation: 1:10\nImmunohistochemistry: 1:250-1:500\nImmunocytochemistry: 1:100-1:250\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

H1831-25N

Size

100ul

Applications

ELISA, FC, ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant.

Isotype

IgG

References

1. Ungewickell E, EMBO J. 4(13A):3385-91, 1985. 2. Matsui H, et al. Mol Cell. 25(1):99-112, 2007. 3. Deichmann M, et al. Int J Oncol 25(2):259-68, 2004.

Additional Info

Recognizes human Hsp73. Species Crossreactivity: Mouse and rat.