Heat Shock Cognate Protein 71 (HSC71, HSPA8)

The 70kD heat shock proteins (HSP70s) are a highly conserved family of stress response proteins. The HSP70 family of proteins contains both heat/stress inducible and constitutively expressed members known as heat shock cognate proteins. Heat Shock 70 kD Protein 8 (HSPA8), also known as Heat Shock Cognate Protein 71 (HSC71), HSC70, HSP73, HSPA10 and LPS-associated protein 1 (LAP1), is a 646 amino acid (aa) heat shock cognate protein. Many HSPs function as molecular chaperones, facilitating the folding of other cellular proteins. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSPA8 is a ubiquitously expressed protein and rapidly translocates from the cytoplasm to the nucleus in response to heat shock. Two alternatively spliced variants have been identified. This antibody detects isoform 1 but not isoform 2 (HSC54). Human HSPA8 shares greater than 99 % identity with mouse and rat HSPA8. \n\nApplications:\nWestern Blot: An antibody concentration of 1.0ug/ml is recommended. \nOptimal dilutions should be determined by each laboratory for each application. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1831-28

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Detects endogenous human, mouse, and rat HSPA8/HSC71, Isoform 1 in Western Blots. Does not crossreact with recombinant human HSPA1A /HSP70, HSPA2, HSPA6, GRP75 or GRP78 in Western Blots.