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Heat Shock Factor 1 (Heat Shock Factor Protein 1, HSF 1, HSF1, Heat Shock Transcription Factor 1, HSTF 1, HSTF1)

Cat no: H1830-44L

Heat Shock Factor 1 (Heat Shock Factor Protein 1, HSF 1, HSF1, Heat Shock Transcription Factor 1, HSTF 1, HSTF1)

HSF1 is the major heat shock transcriptional factor that binds heat shock element (HSE) in the promoter of heat shock proteins (Hsps) and controls rapid Hsp induction in cells subjected to various environmental stresses. HSF1 gene belongs to a family of vertebrate HSF transactivators, and encodes the orthologous protein found across the eukaryotic phyla. HSF1 regulates HSP synthesis and activates hsp gene transcription. Under normal conditions, cellular HSF1 exists in a transcriptionally repressed state. Induction of transcriptional activity by heat shock then results in the conversion of HSF1 from inactive monomer to a DNA-binding trimer. human HSF1 gene is localized on chromosome 8q24.3.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMCF7 cells\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-44L

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. XianZhong, X. et al. The EMBO Journal. 18:5943

Additional Info

Recognizes human HSF1.

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