Heat Shock Factor 2 (HSF2)

HSF2 belongs to a family of Heat Shock transcription Factors (HSFs) that bind to highly conserved heat shock elements (HSEs) in the promoter regions of heat shock genes and regulate the expression of heat shock proteins (Hsps). Multiple HSFs exist in higher eukaryotes whereas yeast and Drosophila have only a single HSF. HSF1, HSF2, HSF3 have been reported in chicken, HSF1 and HSF2 in mouse and HSF1, HSF2, and HSF4 in human. Most HSFs have several common functional motifs whose amino acid sequences are highly conserved. At the N-terminus of all HSFs there is a DNA binding domain essential for binding to the HSE. Hydrophobic repeats located adjacent to the DNA binding domain are essential for the formation of active trimers which enable all HSFs to specifically bind to DNA. Another short hydrophobic repeat located towards the C-terminal region of most HSFs is thought to be necessary for suppression of trimerization except in the case of yeast HSF and human HSF4. The mouse HSF1 exists as two isoforms which arise from alternative splicing of the HSF1 pre-mRNA. In higher eukaryotes, HSF1 is predominantly found in a diffuse cytoplasmic and nuclear distribution in unstressed cells. On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution (2). Similar to HSF1, murine HSF2 also exist as two isoforms, the smaller HSF2-beta isoform and the longer HSF2-alpha which has been shown to be more transcriptionally active. Constitutive binding of HSF2 to HSE has been found with spermatogenic cells post-implantation embryos and embryonal carcinoma cells in mouse and these observations suggest that HFS2 may have roles in differentiation and development.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunoblot Analysis: 0.5-4ug/ml of this lot detected HSF2 in RIPA lysates from HeLa cells.\nImmunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate HSF2 from HeLa, K562 and NIH/3T3 RIPA lysates.\nImmunocytochemistry: Has been reported by an independent laboratory to show positive immunostaining for HSF2 in HeLa, K562 and NIH/3T3 cells fixed with 2% paraformaldehyde.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H1832-20B

Size

200ug

Applications

ICC, IP, WB

Hosts

Rat

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Mathew, A., et al., Mol. Cell Biol. 21: 7163-7171, 2001.\n2. Mathew, A., et al., Mol. Cell Biol. 18: 5091-5098, 1998.\n3. Sarge, K.D., et al., Biol. Reprod. 50: 1334-1343, 1994.

Additional Info

Recognizes HSF2, Mr ~72kD. Species Crossreactivity: Human, mouse, rat, bovine and yeast.