Heat Shock Protein 104, Recombinant, Saccharomyces cerevisiae (HSP104)

Hsp104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 can protect yeast cells against high temperature and high concentration of ethanol but mutation studies have shown this protein is not required for normal growth. Hsp104 was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. Recombinant HSP-104 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 908 amino acids and having a molecular mass of 102kD.\n\nStability: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

H1833-01S

Size

10ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 100mM sodium chloride, 2mM EDTA and 5% glycerol.

Purity

(same/more than) 90% as determined by:\n(a) Analysis by RP-HPLC.\n(b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.

References

1. Chlamydia Heat Shock Protein 60 Induces Trophoblast Apoptosis through TLR4.\n\nJ Immunol 2006 Jul 15;177(2):1257-63\n\n2. High-Affinity Interactions between Peptides and Heat Shock Protein 70 Augment CD8+ T Lymphocyte Immune Responses.\n\nJ Immunol 2006 Jul 15;177(2):1017-27\n\n3. Preclinical pharmacokinetics and metabolism of a novel diaryl pyrazole resorcinol series of heat shock protein 90 inhibitors.\n\nMol Cancer Ther 2006 Jun;5(6):1628-37\n\n4. The function of the beta3 interactive domain in the small heat shock protein and molecular chaperone, human alphaB crystallin.\n\nCell Stress Chaperones 2006 Summer;11(2):187-97\n\n5. Overexpression of inducible heat shock protein 70 and its mutants in astrocytes is associated with maintenance of mitochondrial physiology during glucose deprivation stress.\n\nCell Stress Chaperones 200 6 Summer;11(2):180-6\n\n6. Overexpressed heat shock protein 70 protects cells against DNA damage caused by ultraviolet C in a dose-dependent manner.\n\nCell Stress Chaperones 2006 Summer;11(2):162-9