Heat Shock Protein 20 (HSP20, FLJ32389, Heat Shock Protein alpha-Crystallin-related B6, Heat Shock Protein beta 6, Hspb6, Heat Shock 20kD Like-protein p20)

Applications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1830-48E

Size

50ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, Fel, GP, Prc, Rabbit, Sheep

Form

3% BSA, 0.1M Phosphate. pH 7.0

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Tessier DJ et al. The small heat shock protein (HSP) 20 is dynamically associated with the actin cross-linking protein actinin. J Surg Res 111:152-7 (2003). <PUBMED:12842460>Kozawa O et al. HSP20, low-molecular-weight heat shock-related protein, acts extracellularly as a regulator of platelet functions: a novel defense mechanism. Life Sci 72:113-24 (2002). <PUBMED:12417245>Beall A et al. The small heat shock-related protein, HSP20, is phosphorylated on serine 16 during cyclic nucleotide-dependent relaxation. J Biol Chem 274:11344-51 (1999). <PUBMED:10196226>Brophy CM et al. Phosphorylation of the small heat shock-related protein, HSP20, in vascular smooth muscles is associated with changes in the macromolecular associations of HSP20. J Biol Chem 274:6324-9 (1999). <PUBMED:10037721>Beall AC et al. Cyclic nucleotide-dependent vasorelaxation is associated with the phosphorylation of a small heat shock-related protein. J Biol Chem 272:11283-7 (1997). <PUBMED:9111032>Kato K et al. Purification and characterization of a 20-kDa protein that is highly homologous to alpha B crystallin. J Biol Chem 269:15302-9 (1994). <PUBMED:8195168>

Additional Info

Species Crossreactivity: Crossreacts with Human, Mouse, Rat, bovine, Cat, canine, Guinea pig, Pig, Rabbit and Sheep.