Heat Shock Protein 20 (HSP20)

Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and alphaB-crystallin and may form different hetercomplexes with these proteins. The specific physiologic function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. Hsp20 can be phosphorylated in vitro by cAMP-dependant protein kinase (PKA) and cGMP-dependant protein kinase (PKG). Phosphorylated Hsp20 is proposed to interact with monomeric actin and alpha-actinin whereas dephosphorylated Hsp20 binds polymeric actin filaments. Phosphorylated Hsp20 is also associated with vasorelaxation. Dissociated Hsp20, but not aggregated Hsp20, suppresses platelet aggregation, and human platelets show specific binding sites for Hsp20. Elevated levels of dissociated Hsp20 have also been found in the plasma of cardiomyopathic hamsters. Hsp20, which immediately responds to pathological events, may act extracellularly as a regulator of platelet functions.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions:\nWestern Blot: 1ug/ml detected HSP20 in RIPA lysates from rabbit skeletal muscle and mouse heart cytosol.\nImmunoprecipitation: Reported to immunoprecipitate HSP20 from rat heart extract and lysates of bovine carotid artery smooth muscle.\nImmunohistochemistry: Reported to detect HSP20 in blood vessels fixed with paraffin.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-48D

Size

100ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Rabbit

Form

Supplied as a liquid in 0.1M PBS, pH 7.0, 0.1% sodium azide, 30mg/ml BSA, before the addition of glycerol to 50%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

US Biological application reference: Cross, B.E. et al., Reproduction (2007) 133: 807-817. 1. Beall, K.C., et al., J. Biol. Chem. 272: 11,283-11,287 (1997). 2. Kato, K., et al., J. Biol. Chem. 269: 11,274-11,278 (1994).

Additional Info

Recognizes human HSP20, Mr ~20kD. Species Crossreactivity: mouse, rabbit, rat and bovine