Heat Shock Protein 20, Recombinant, Human (HSP20)

Dimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nImmunological Activity:\nImmunoreactivity is confirmed by reaction with monoclonal mouse antibodies against HSP-20.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of HSP-20.\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm. \n2. Analysis by RP-HPLC, using a calibrated solution of Human Heat Shock Protein as a Reference Standard.\n\nReconstitution:\nReconstitute the lyophilized HSP-20 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1830-48A

Size

2ug

Form

Supplied as a lyophilized powder in 20mM Tris-acetate, pH 7.6, 10mM sodium chloride, 0.1mM EDTA, 0.1mM PMSF, 15mM A-mercaptoethanol.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Transducible heat shock protein 20 (HSP20) phosphopeptide alters cytoskeletal dynamics. Dreiza CM, Brophy CM, Furnish EJ, von Rechenberg M, FASEB J 2004 Dec 14; 2. Comparison of the small heat shock proteins alphaB-crystallin, MKBP, HSP25, HSP20, and cvHSP in heart and skeletal muscle. Golenhofen N, Perng MD, Drenckhahn D, Histochem Cell Biol 2000 Nov;122(5):415-425 3. Transducible recombinant small heat shock-related protein, HSP20, inhibits vasospasm and platelet aggregation. McLemore EC, Tessier DJ, Furnish EJ, Stone WM, Surgery 2004 Sep;136(3):573-8 4. Inactivity-induced modulation of Hsp20 and Hsp25 content in rat hindlimb muscles. Huey KA, Thresher JS, Roy RR, Muscle Nerve 2004 Jul;30(1):95-101 5. Transduction of peptide analogs of the small heat shock-related protein HSP20 inhibits intimal hyperplasia. Tessier DJ, Komalavilas P, Kent CK, Joshi L, J Vasc Surg 2004 Jul;40(1):106-14 6. Heat shock-related protein 20 (HSP20) in cardiomyocytes. Brophy CM, Komalavilas P, Circ Res 2004 May 28;94(10):e85.