Heat Shock Protein 25, Recombinant, Mouse (HSP25)

Mouse Hsp25, human Hsp27, and alpha-beta-crystallin are part of a diverse family of small heat shock proteins (sHsps) which are produced in all organisms. The overall homology between the different sHsps is low and they are grouped together based on conserved sequences in the C-terminal half of the protein and short conserved phenylalanine-rich stretches near the N terminus. Mammalian sHsps are expressed constitutively under physiological conditions but stress factors such as heat shock induce a strong up-regulation of protein levels by 10-20-fold to maximum concentrations of 0.1% of the cellular protein. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. sHsps all share the striking feature of forming high molecular weight oligomeric complexes of variable size. The quaternary structure of sHsps is essential for their function and regulation of activity but its basic properties are still rather poorly understood. Data indicates that Hsp25 is a dynamic tetramer of tetramers with a unique ability to refold and reassemble into its active quaternary structure after denaturation (1). Current studies demonstrate that Hsp25 helps facilitate the glutathione-redox cycle by enhancing glutathione utilization and maintaining the cellular glutathione pool in favor of the reduced states (2).\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: Dilute in an SDS containing sample buffer. Heat at 100 degrees C for 5 minutes prior to use. On a 15-well minigel system, loading 50ng-100ng of protein per lane should be sufficient.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

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SPECIFICATIONS

Catalog Number

H1830-49B

Size

20ug

Applications

ELISA, WB

Form

Supplied as a liquid in 1X Dulbecco

Purity

(same/more than) 90% as determined by SDS-PAGE analysis.

References

1. Ehrnsperger, M., Lilie, H., Gaestel, M., Buchner, J., J. Biol. Chem. 274: 14867-14874 (1999). 2. Baek, S.H., Min, J.N., Park, E.M., Han, M.Y., Lee, Y.S., Lee, Y.J. and Park, Y.M., J. Cell Physiol 183: 100-107 (2000). 3. Laemmli, U.K., Nature 277: 680-685 (1970).