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Heat Shock Protein 27, aa1-205, Recombinant Human, His-Tag (HSP27)

Cat no: H1834-05E

Heat Shock Protein 27, aa1-205, Recombinant Human, His-Tag (HSP27)

Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant human HSP27 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.\n\nSource:\nRecombinant human HSP27 protein, fused to His-tag at N-terminus, was expressed in E. coli \n\nAA sequence:\nMGSSHHHHHH SSGLVPRGSH MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE \nGTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1834-05E

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH8.0, 1mM DTT, 10% glycerol.

Purity

~95% (SDS-PAGE) Purified by immunoaffinity chromatography.

References

1.Friedman MJ., et al. (2009) J Biol Chem. 284(41):27944-51. 2.Brunet Simioni M., et al. (2009) Oncogene. 28(37):3332-44.

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