Heat Shock Protein 27 (HSP27) (Biotin)

Human HSP27, mouse HSP25 and alpha-beta-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. HSP27 is believed to exist mainly as oligomers of as many as 8

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SPECIFICATIONS

Catalog Number

H1834-05C

Size

50ug

Applications

ELISA, ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, NHP

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2, and 0.09% sodium azide before the addition of glycerol to 40%. Labeled with Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Ehrnsperger, M., et al., EMBO J. 16: 221-229 (1997). 2. Benndorf, R., et al., J. Biol. Chem. 269: 20,780-20,784 (1994). 3. Mehlen, P., et al., Biochem. Biophys. Res. Commun. 241: 187-192 (1997). 4. Pandey, P., et al., Oncogene 19: 1975-1981 (2000). 5. Ciocca, D.R., et al., Cancer Res. 42: 4256-4258 (1982). 6. Ciocca, D. R., et al., J. Clin. Endocrinology Metabolism 60: 137-143 (1985). 7. Adams, D.J., et al., Cancer Res. 43: 4297-4301 (1983). 8. Adams, D.J. and McGuire, W.L., Cancer Res. 45: 2445-2449 (1985). 9. Grandour-Edwards, R., et al., Modern Pathology 8: 160-164 (1995). 10. Trautinger, F., et al., J. Photochem. Photobio. B. Biology 39: 90-95 (1997). 11. Munoz de Toro, M.M. and Luque, E.H., J. Steroid Biochem. Molec. Biol. 60: 277-284 (1997). 12. Piotrowicz, R.S., et al., FASEB J. 12: 1481-1490 (1998). 13. Tezel, G. and Wax, M.B., Invest. Ophthalmol. Vis. Sci. 40: 2660-2667 (1999). 14. Sato, J.-I. and Kim, S.U., J. Neuroscience Res. 41: 805-818 (1995). 15. Jonak, C., et al., Br. J. Dermatol. 147(1): 13-19 (2002).

Additional Info

Recognizes a ~27kD protein, corresponding to the apparent molecular mass of HSP27 on SDS-PAGE immunoblots. \nSpecies Crossreactivity: Human, monkey and fish (rainbow trout). Does not exhibit reactivity with rodent HSP25, alphaA, alphaB or beta-crystallin proteins.