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Heat Shock Protein 40 (HSP40, HDJ1)

Cat no: H1834-15

Heat Shock Protein 40 (HSP40, HDJ1)

HSP40, also known as HDJ1 (9), is a basic mammalian 40kD heat shock protein which is homologous to the bacterial heat shock protein, DnaJ, and yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (1,2,3,4). Like many heat shock proteins, HSP40 is inducible by heat, transient metals and azetidine carboxylic acid in mammalian and avian cells (2). In heat shocked HeLa cells, HSP40 translocates rapidly from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (1). This suggests that HSP40 co-localizes with HSP70 and together they repair denatured proteins in the nuclei and nucleoli of heat shocked cells (1). This is analogous to the functions of bacterial DnaJ and DnaK which have been shown to act together in dissociating the bacteriophage lambda protein complexes (5,6) and monomerizing the Rep A dimer (7).\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:5000 (Colorimetric)\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by researcher.\n\nPositive Controls:\nRecombinant Human Hsp40 Protein\nHeLa Heat Shocked Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1834-15

Size

25ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, NHP, Prc, Rabbit, Sheep

Form

Supplied as a liquid in 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Hattori, H., et al., Cell Structure and Function 17: 77-86 (1992). 2. Ohtsuka, K., et al., Biochem. Biophys. Res. Commun. 166: 642-647 (1990). 3. Bardwell, J.C.A., et al., J. Biol. Chem. 261: 1782-1785 (1986). 4. Ohku, M., et al., J. Biol. Chem. 261: 1778-1781 (1986). 5. Georgopoulos, C., et al., Stress Proteins in Biology and Medicine (R.I. Morimoto, A. Tissieres, and C. Georgopoulos, eds.) Cold Spring Harbor Lab Press pp191-221 (1990). 6. Zylicz, M., et al., EMBO. J. 8: 1601-1608 (1989). 7. Wickner, S., et al., Proc. Natl. Acad. Sci. 88: 7903-7907 (1991). 8. Meacham, G.C., et al., EMBO J. 18: 1492-1505 (1999). 9. Ohtsuka, K., Biochem. Biophys. Res. Commun. 197: 235-240 (1993).

Additional Info

Detects a 40kD protein, corresponding to the apparent molecular mass of HSP40 on SDS-PAGE immunoblots. It does not detect DnaJ in E. coli lysates. Species Crossreactivity: Human, monkey, mouse, rat, hamster, guinea pig, rabbit, canine, bovine, sheep, porcine, chicken and fish.

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