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Heat Shock Protein 47 (HSP47, Colligin, gp46, AsTP3, CBP1, CBP2, Collagen-binding protein 1, Collagen-binding protein 2 precursor, Colligin 1, Colligin 2, gp46, HSP47, arthritis-related antigen RA-A47, SERPINH2)

Cat no: H1834-20E

Heat Shock Protein 47 (HSP47, Colligin, gp46, AsTP3, CBP1, CBP2, Collagen-binding protein 1, Collagen-binding protein 2 precursor, Colligin 1, Colligin 2, gp46, HSP47, arthritis-related antigen RA-A47, SERPINH2)

Heat shock protein 47(HSP47), also known as Colligin or Serpin H1, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein acts as a molecular chaperone facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting the transport of correctly folded-molecules from the ER to Golgi apparatus. Recombinant human HSP47/Colligin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.\n\nSource: \nHuman, His-tagged, Recombinant, E. coli,18-418aa. \n\nAA Sequnce: \nMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA EVKKPAAAAA PGTAEKLSPK AATLAERSAG LAFSLYQAMA KDQAVENILV SPVVVASSLG LVSLGGKATT ASQAKAVLSA EQLRDEEVHA GLGELLRSLS NSTARNVTWK LGSRLYGPSS VSFADDFVRS SKQHYNCEHS KINFRDKRSA LQSINEWAAQ TTDGKLPEVT KDVERTDGAL LVNAMFFKPH WDEKFHHKMV DNRGFMVTRS YTVGVMMMHR TGLYNYYDDE KEKLQIVEMP LAHKLSSLII LMPHHVEPLE RLEKLLTKEQ LKIWMGKMQK KAVAISLPKG VVEVTHDLQK HLAGLGLTEA IDKNKADLSR MSGKKDLYLA SVFHATAFEL DTDGNPFDQD IYGREELRSP KLFYADHPFI FLVRDTQSGS LLFIGRLVRP KGDKMRDEL\n\nStorage and Stability:\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1834-20E

Size

100ug

Applications

WB

Form

Supplied as a liquid in 20mm Tris-HCl buffer, pH 8.0, 10% glycerol.

Purity

(same/more than) 90% (SDS-PAGE)

References

1. Mohammed Tasab., et al. (2000) The EMBO Journal. 19(10): 2204

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