Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60)

The human Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65kD major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1billion years) and this fact together with the high degree of homology between the divergent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species. The common characteristics of the Hsp60

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SPECIFICATIONS

Catalog Number

H1830-77B

Size

100ug

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, NHP, Prc, Rabbit, Yeast/Fun

Form

Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Jindal, S., Dudani, A.K., Singh, B., Harley, C.B., Gupta, R.S. (1989) Molecular and \nCellular Biology 9: 2279-2283. \n2. Nielsen, K.L., Mclennan, N., Masters, M., Cowan, N.J. (1999) J. Bacteriol. 181: 5871-\n5875. \n3. Samali, A., Cia, J.Y., Zhivotovsky, B., Jones, D.P., Orrenius, S. (1999) EMBO J. 18: 2040-\n2048. \n4. Van Roon, J.A-G., van Eden, W., van Roy, J.L.A.M., Lafeber, F.J.P.G., Bijisma, J.W.J. \n(1997) J Clin. Invest 100: 459-463. \n5. La Verda, D., Kalayoglu, M.V., Byrne, G.I. (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. \n6. Boog, C.J.P., de Graeff-Meeder, E.R., Lucassen, M.A., van der Zee, R., Voorhorst-Ogink, \nM.M., van Kooten, J. S., Geuze, H.J., van Eden, W. (1992) J. Exp. Med. 175: 1805-1810. \n7. Zhang, Y., Ohashi, N., Lee, E.H., Tamura, A., Rikihisa, Y. (1997) FEMS Immunology \nand Medical Microbiology 18: 39-46. \n8. Belles, C., Kuhl, A., Nosheny, R., Carding, S.R. (1999) Infect. Immun. 67: 4191-4200. \n9. Munoz, M. G., et al. (1996) Eur. Soc. Hum. Reprod. and Em. 11: 2600-2738. \n10. Kligman, I., Grifo, J.A., Witkin, S.S. (1996) Eur. Soc. Hum. Repro. & Em. 11: 2736-2738 \n

Additional Info

Recognizes human Hsp60 at ~60kD. Epitope: located within aa 383-419 of human Hsp60 (aa 356-393 of the mycobacterial Hsp60). \nSpecies Crossreactivity: Mouse, rat, bovine, C. elegans, chicken, canine, guinea pig, hamster, monkey, porcine, rabbit, plant (spinach, tomato), Borellia, E. coli (GroEL), Helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, white fly, Yersinia entercolitica and yeast.