Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60)

The human Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65kD, a major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrail matrix and the plant Hsp60, is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1billion years) and this fact together with the high degree of homology between the divergent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (1). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its co-chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (2). Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase-3 by cytochrome c and dATP in an ATP-dependent manner (3). Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, artherosclerosis and chlamydial disease.\n\nApplications: \nWestern Blot (ECL): 1:10,000 \nImmunoprecipitation: 1:100 \nOptimal dilutions to be determined by researcher.\n\nPositive Controls:\nRecombinant Human Hsp60 Protein \nHeLa Cell Lysate

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SPECIFICATIONS

Catalog Number

H1830-77E

Size

50ug

Applications

IP, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Dro/Arth, GP, NHP, Prc, Rabbit, Sheep, Xen/Amph

Form

Serum

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Jindal, S., Dudani, A.K., Singh, B., Harley, C.B. and Gupta, R.S. (1989) Molecular and \nCellular Biology 9: 2279-2283. \n2. Nielsen, K.L., Mclennan, N., Masters, M and Cowan, N.J. (1999) J. Bacteriol. 181: 5871-\n5875. \n3. Samali, A., Cia, J.Y., Zhivotovsky, B., Jones, D.P., and Orrenius, S. (1999) EMBO J. 18: \n2040-2048. \n

Additional Info

This antibody detects an ~60kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, beluga, bovine, canine, chicken, Drosophila, E. coli (GroEL), fish (rainbow trout), guinea pig, \nhamster, monkey, M. bovis (Hsp65), porcine, rabbit, sheep and Xenopus.