Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60)

The 60kDa heat shock protein (HSP60) is a highly conserved protein that act as a chaperone involved in correct folding of newly synthesized proteins (1-2). Mainly expressed in mitochondria, HPS60 has also been found in the cytosol of normal heart and muscle cells. In normal cardiac myocytes, cytosolic HSP60 complexes with Bax, Bak and Bcl-XL (3). While over expression of HSP60 prevents apoptosis in cardiac cells; is has also been shown that HSP60 accelerated the activation of Caspase 3 during apoptosis (4-5). Therefore, the role of HSP60 in apoptosis may be both pro- and anti-apoptotic, depending on the cell type, or apoptotic stimulus. \n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:5000 \nImmunohistochemistry: 1:100-1:250 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H1834-25M

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant.

Isotype

IgG

References

1. Prasad YM, et al. Mol Cell Biol (8):3979-86, 1990 2. Czarnecka AM, et al. Cancer Biol Ther. (7):714-20, 2006 3. Patriarca EJ, Exp Cell Res. (1):57-64, 1990 4. Davies EL, et al. Clin Exp Immunol. (1):183-9, 2006

Additional Info

Recognizes human Heat Shock Protein 60. Species Crossreactivity: Mouse and rat.