Heat Shock Protein 60 (HSP60, HSP-60, 60kD Chaperonin, 60kD Heat Shock Protein Mitochondrial, Chaperonin 60, CPN60, HSPD1, HuCHA60, Mitochondrial Matrix Protein P1, P60 Lymphocyte Protein)

In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. Hsp60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60 (1-3). Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo- oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its co- chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co- chaperonin, GroES (5). Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9). \n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml detects Hsp60 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot using goat anti-mouse (HRP) as a secondary antibody. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-77G

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Rabbit

Form

Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Cho S., et al. (2007). Mol Cell Biol 27(1): 368-83, 2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555, 3. Luo S., et al (2006) 26 (15): 5688-974, 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314, 5. Koomagi R., et al. (1999) Anticancer Res. 19:4333- 4336, 6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949, 7. Dong D., et al. (2005) Cancer Res. 65(13): 5785-91.

Additional Info

Recognizes human Hsp60. Species Crossreactivity: mouse, rat, bovine, canine, chicken, rabbit.