Heat Shock Protein 70 (HSP70)(Alexa Fluor(R) 647 Conjugate)

HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP and co-chaperone dependent manner (1). HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop and p23 (2,3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1,4). When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSP70/HSP90 go beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1,3). They also play a role in vesicle formation and protein trafficking (2). \n\nApplications: \nSuitable for use in Flow cytometry and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nFlow cytometry: Add 10ul to 500,000 cells in 90ul PBS/0.5% BSA.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1831-14

Size

500ul

Applications

FC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in PBS (pH 7.2), less than 0.1% sodium azide, 2mg/ml BSA. Conju- \ngated to Alexa Fluor(R) 647. F/P ratio: 2-6.

P Type

Pab

Purity

Purified by protein A and peptide affinity chromatography.\n

Isotype

IgG

References

(1) Nollen, E.A. and Morimoto, R.I. (2002) J. Cell Sci. 115, 2809

Additional Info

Recognizes endogenous levels of total human HSP70 protein. This antibody does not cross-react with other HSPs. Species Cross-reactivity: mouse, rat and monkey