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Heat Shock Protein 70 (HSP70)

Cat no: H1830-95D

Heat Shock Protein 70 (HSP70)

Hsp70 is a human heat shock protein. Hsp70 is an important part of the cell's machinery for protein folding, and help to protect cells from stress. In most species, there are many proteins that belong to the hsp70 family. Some of these are only expressed under stress conditions, while some are present in cells under normal growth conditions and are not heat-inducible. Hsp70 can be found in different cellular compartments (nuclear, cytosolic, mitochondrial, endoplasmic reticulum, etc...), and the chaperone function of hsp70 is required for protection against stress-induced apoptosis. \n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:2000\nImmunohistochemistry: 1:100-1:200 Validated on paraffin-embvedded sections of human eccrine gland incubated with H1830-95D for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-95D

Size

50ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1.Lewis MJ, et al., (1985) EMBO J 4:3137-3143 Gething M-J, et al., (1992) Nature 355:33-45 Dick D.M, et al., (2000) Mol Cell Biol 20(19):7146-7159

Additional Info

Recognizes human Hsp70.

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