Heat Shock Protein 70 (HSP70)

Hsp70 genes encode abundant heat-inducible 70kD hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can \nbe stimulated by binding to unfolded proteins and synthetic peptides (2). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was \nfound to reside in an N-terminal fragment of 44kD which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATPbinding domain displays multiple features of nucleotide binding proteins (4). When cells are subjected to metabolic stress (e.g., heat shock) a member of the hsp 70 family, hsp 70 hsp72), is expressed; hsp 70 is highly related to hsc70 (>90% sequence identity). Constitutively expressed hsc70 rapidly forms a stable complex with the highly inducible hsp70 in cells \nfollowing heat shock. The interaction of hsc70 with hsp 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on hsc70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (5). \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot:1:5000 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-95T3

Size

100ul

Applications

WB

Hosts

Rabbit

Form

Supplied as a lyophilized powder. Reconstitute with 100ul sterile water.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1):1-17. 2. Rothman J. (1989), Cell 59:591-601. 3. DeLuca-Flaherty et al. (1990) Cell 62:875-887. 4. Bork P., Sander C., and Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89:7290-7294. 5. Brown C.L. et al. (1996) J. Biol. Chem. 271(2):833-840.

Additional Info

Recognizes Hsp70. Species Crossreactivity: Rainbow trout. This antibody is specific for salmonid Hsp70 (tested on salmon and brooktrout). It does not cross-react with Hsc70. It does not detect Hsp70 in other species.