Heat Shock Protein 70 (HSP70, HSP72) (Biotin)

The heat shock protein HSP70 is part of the HSP70 family which contains a number of highly-related protein isoforms ranging in size from 66kD to 78 kDa. The 70 kD heat shock cognate protein, hsc70, is closely related biochemically and biologically to hsp70 and is part of the HSP70 family. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution (2). Members of the HSP70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an ATP-dependent manner. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (3). Recent data has demonstrated that BAG-1 which possesses a ubiquitin-like domain at its amino terminus and which has been shown to associate with the 26S proteosome in HeLa cells, modulates the chaperone activity of hsc70 and HSP70. These findings reveal a role of BAG-1 as a physical link between the hsc70/HSP70 chaperone system and the proteosome (4). There is also experimental data which shows that the ATPase domain and the substrate binding domain of hsp70 (or hsc70) cooperate and form a co-chaperone-chaperone complex with the synaptic vesicle cysteine string protein (csp) which is essential for normal neurotransmitter release (5).\nApplications: \nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot (Colorimetric): 1:1000\nOptimal dilutions to be determined by researcher.\n\nPositive Controls:\nRecombinant Human Hsp70 Protein: H1830-92\nHeLa Heat Shocked Cell Lysate: H1838-01\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H1831-12

Size

50ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, NHP, Prc, Rabbit, Sheep

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Welch, W.J., Suhan, J.P., J. Cell Biol. 103: 2035-2050 (1986). 2. Tavaria, M., et al., Cell Stress & Chaperones 1: 23-28 (1996). 3. Fink, A.L., Physiol. Rev. 79: 425-449 (1999). 4. Luders, J., et al., J. Biol. Chem. 275: 4613-4617 (2000). 5. Stahl, B., et al., Eur. J. Cell Biol. 78: 375-381 (1999). 6. Moon, I.S., et al., Cerebral Cortex 11: 238-248 (2001). 7. Dressel, R., et al., J. Immunol. 164: 2362-2371 (2000). 8. Pockley, A.G., et al., Immunol. Invest. 27: 367-377 (1998). 9. Anthanassiadou, P., et al., Eur. J. Can. Prev. 7: 225-231 (1998). 10. Misaki, T., et al., Clin. Exp. Immun. 98: 234-239 (1994). 11. Galan, A., et al., J. Biol. Chem. 275: 11,418-11,424 (2000). 12. Kondo, T., et al., J. Biol. Chem. 275: 8872-8879 (2000). 13. Kilgore, J., et al., J. App. Physiol. 76: 598-601(1994). 14. Davies, R.L., et al., Amer. J. Pathol. 155: 1901-1913 (1999). 15. Hofmann, K., Wood, S.W., Brinton, C.C., Montibeller, J.A., Finn, F.M., PNAS 77: 4666-4668 (1980).

Additional Info

Recognizes human Hsp70 protein at 70kD, corresponding to the apparent MW of inducible HSP. Does not crossreact with the constitutive Hsc70 (Hsp73). Species Crossreactivity: monkey, rabbit, rat, mouse, hamster, guinea pig, bovine, sheep, canine, porcine, chicken, teal, beluga, carp.