Heat Shock Protein 70 (HSP70, HSP72)

The heat shock protein, Hsp70 is part of the Hsp70 family which contains a number of highly-related protein isoforms ranging in size from 66 kD to 78 kD. The 70 kD heat shock cognate protein, Hsc70 is closely related biochemically and biologically to Hsp70 and is part of the Hsp70 family. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an ATP-dependent manner. The molecular chaperones of the Hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding and the binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. Recent data has demonstrated that BAG-1 which possesses a ubiquitin-like domain at its amino terminus and which has been shown to associate with the 26S proteosome in HeLa cells, modulates the chaperone activity of Hsc70 and Hsp70. These findings reveal a role of BAG-1 as a physical link between the Hsc70/hsp70 chaperone system and the proteasome. There is also experimental data which shows that the ATPase domain and the substrate binding domain of hap70 (or hsc70) cooperate and form a cochaperone-chaperone complex with the synaptic vesicle cysteine string protein (csp) which is essential for normal neurotransmitter release \n \nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot (ECL): 1:30,000 \nImmunoprecipitation: 1:100 \nELISA: 1:1000 \nOptimal dilutions to be determined by researcher.\n \nPositive Controls:\nRecombinant Human Hsp70 Protein,\nHeat Shocked HeLa Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1834-35B

Size

50ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, GP, NHP, Prc, Sheep

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% azide, 50% glycerol

P Type

Pab

Purity

Purified by Protein A affinity chromatography

Isotype

IgG

References

1. Tavaria, M., et al., Cell Stress & Chaperones 1: 23-28 (1996). 2. Fink, A.L., Physiol. Rev. 79: 425-449 (1999). 3. Luders, J., et al., J. Biol. Chem. 275: 4613-4617 (2000). 4. Stahl, B., et al., Eur. J. Cell Biol 78: 375-381 (1999). 5. Hunt, C. & Morimoto, R.I., PNAS USA. 82: 6455-6459 (1985). 6. Pockley, A.G., et al., Immunolical Investigations 27(6): 367-377 (1998). 7. Nollen, E.A.A., et al., Mol. Cell Biol. 20: 1083-1088 (2000). 8. Warrick, J.M., et al., Nature Genetics 23: 425-428 (1999). 9. Trentin, G.A. et al., J. Biol. Chem. 276(16): 13,087-13,095 (2001). 10. Ianaro, A., et al., FEBS Lett. 508: 61-66 (2001). 11. Hung, T.H., et al., Am. J. of Pathol. 159(3): 1031-1043 (2001). 12. Locke, M., Cell Stress & Chaperones 5(1): 45-51 (2000). 13. Ianaro, A., et al., FEBS Lett 508(1): 61-66 (2001). 14. Downs, C.A., et al., Free Radic. Biol. Med. 33(4): 533-543 (2002). 15. Tarozzo, G., et al., Eur. J. Neurosci. 15(10): 1663-1668 (2002).

Additional Info

Detects an ~70kD protein, corresponding to the apparent molecular mass of Hsp70 on SDS-PAGE immunoblots. Specific for the inducible form of Hsp70 (Hsp72) and does not crossreact with the constitutive Hsc70 (Hsp73) or DnaK. Species Crossreactivity: Human, mouse, rat, beluga, bovine, canine, fish (carp), guinea pig, hamster, monkey, porcine, sheep and coral.