Heat Shock Protein 90 alpha, BioAssay(TM) Kit (Hsp90a)

The Hsp90a BioAssay(R) Kit is designed for identification of Hsp90a inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant Hsp90a. Hsp90a is a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90a functions has been shown to play a role in tumorigenesis and disease progression. The Hsp90a inhibitor screening assay kit comes in a convenient 96-well format, with purified Hsp90a enzyme, fluorescently labeled geldanamycin and Hsp90a assay buffer for 100 enzyme reactions. The key to the Hsp90a BioAssay(TM) Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required forHsp90a reactions. The fluorescently labeled geldanamycin is incubated with a sample containing Hsp90a enzyme to produce a change in fluorescent polarization that can then be measured using a fluorescence reader. \n\nKit Components:\n1. Hsp90a Recombinant Enzyme: 1x70ug. Store at -70 degrees C.\n2. Geldanamycin (FITC) 2.5uM: 1x30ul. Store at -70 degrees C.\n3. 5X Hsp90a assay buffer: 1x4ml. Store at -20 degrees C.\n6. Black, low binding NUNC black microtiter plate: 1 plate. Store at room temperature.\n\nAPPLICATIONS: \nSuitable for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested.\n\nStorage and Stability:\nStore buffer and standard at -20 degrees C. Store enzyme and substrate at -70 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1832-86

Size

96Tests

References

1. Kim J, Felts S, LLauger L, He H, Huezo H, Rosen N, and Chiosis G. J Biomol Screening 2004; 9(5): 375-381. 2. Howes R, Barril X, Dymock BW, Grant K, Northfield CJ, Robertson AGS, Surgenor A, Wayne J, Wright L, James K, Matthews T, Cheung KM, McDonald E, Workman P, Drysdale MJ. Anal Biochem 2006; 350:202-213.