Heat Shock Protein 90, beta (HSP90b)

The 90kD heat shock protein (HSP90) is a molecular chaperone that keeps a target protein in a folding-competent state. Hsp90 is ubiquitously expressed and abundant, and it participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase. In its ATP-bound state, Hsp90 interacts with the co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. Hsp90 is highly conserved between species; mammalian proteins demonstrate 60% amino acid identity with the corresponding yeast and 78% identity with the corresponding Drosophila proteins. Two isoforms of HSP90, alpha and beta, are expressed in the cytosolic compartment.6 The amino acid sequence of human HSP90 alpha is 85% homologous to HSP90 beta, but HSP90 alpha exists predominantly as a homodimer while HSP90 beta exists mainly as a monomer.\n\nApplications:\nSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nELISA: 1:100-1:1000\nImmunoprecipitation: 100ul/test\nWestern Blot: 1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls: HeLa, T47D, A549, and PC-3 cell lysates and rabbit reticulocyte lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1834-55J

Size

1ml

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.1% sodium azide

P Type

Mab

Purity

Supernatant

Isotype

IgG2a,k

References

1. Arlander SJH, et al. J Biol Chem 278(52):52572-52577, 2003. 2. Pearl H, et al. Adv Protein Chem 59:157-186, 2001. 3. Neckers L, et al. Trends Mol Med 8:S55-S61, 2002. 4. Pratt W, Toft D. Exp Biol Med 228:111-133, 2003. 5. Chadli A, et al. PNAS 97(23):12524-12529, 2003. 6. Nemoto T, et al. J Biol Chem 272(42):26179-26187, 1997. 7. Hickey E, et al. Mol Cell Biol 9:2615-2626, 1989. 8. Minami Y, et al. J Biol Chem 266:10099-10103, 1991.

Additional Info

Recognizes beta isoform of human HSP90 at ~90kD. Recognizes purified human HSP90 beta and purified chicken HSP90 alpha by Western Blot and Immunoprecipitation. Does not react with purified human HSP90 alpha in Western Blot.