Heat Shock Protein 90, beta, phosphorylated (Ser254) (Heat Shock Protein HSP 90-beta, HSP90b, HSP 90, D6S182, FLJ26984,Heat Shock 84kD, HSP 84, HSP84, Heat Shock Protein 90kD alpha (Cytosolic) Class B Member 1, HSP90AB1, HSPC2, HSPCB)

The 90kD molecular chaperone family comprises several proteins including the 90kD heat shock protein, Hsp90 and the 94kD glucose-regulated protein, grp94 which are major molecular chaperones of the cytosol and of the endoplasmic reticulum. In mammalian cells there are at least two Hsp90 isoforms, Hsp90 alpha and Hsp90 beta which are encoded by separate genes. Hsp90 is part of the cell's powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steroid receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways. Recent studies indicate that when Drosophila Hsp90 buffering is compromised by e.g., temperature, cryptic variants are expressed and selection can lead to the continued expression of these traits, even if Hsp90 function is restored.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHT29 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1834-55X

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Scheibel, T. et al. PNAS. 96:1297-1302 (1999). 2. Nemoto, T. et al. J. Biol. Chem. 272:26179-26187 (1997). 3. Nemoto, T. et al. Biochem. J. 330:989-995 (1998).

Additional Info

Recognizes human Hsp90b, phosphorylated at Ser254. Species Crossreactivity: mouse and rat.