Heat Shock Protein 90 (HSP90)

The 90kD molecular chaperone family comprises several proteins including the 90kD heat shock protein, Hsp90 and the 94kD glucose-regulated protein, grp94 which are major molecular chaperones of the cytosol and of the endoplasmic reticulum. In mammalian cells there are at least two Hsp90 isoforms, Hsp90alpha and hsp90beta which are encoded by separate genes. The amino acid sequence of human and yeast Hsp90alpha is 85% and 90% homologous to that of Hsp90beta respectively (1). All known members of the Hsp90 protein family are highly conserved. These proteins account for 1-2% of all cellular proteins in most cells. Hsp90 fights the deleterious consequences of protein unfolding, however, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signalling and cell cycle control. In this context several key regulatory proteins have been identified as substrates of Hsp90 (4). It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways. Recent studies indicate that when Drosophilia Hsp90 buffering is compromised by temperature etc., cryptic variants are expressed and selection can lead to the continued expression of these traits, even if Hsp90 function is restored (5).\n \nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot (Colorimetric): 1:1000\nOptimal dilutions to be determined by researcher.\n\nPositive Controls:\nH1831-57: Heat Shock Protein 90, Human (HSP90)\nH1832-85:Heat Shock Protein 90 alpha, Recombinant, Human\nHeLa Heat Shocked Cell Lysate (HSP90a)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H1834-55C

Size

50ug

Applications

WB

Hosts

Rat

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Dro/Arth, GP, NHP, Prc, Rabbit, Sheep

Form

Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Nemoto, T. et al (1998) Biochem. J. 330: 989-995. 2. Scheibel, T., Buchner, J. (1997) in Guidebook to Chaperones, ed. Gething, M.J. (Oxford Univ. Press, Oxford), pp. 147-1503. Scheibel, T., et al (1999) J. PNAS 96: 1297-13024. Scheibel, T. et al (1998) J. Biochem. Pharmacol. 56: 675-682 5. Rutherford, S. L. et al (1998) Nature 396: 336-3426. Lai, B-T., et al (1984) Molecular & Cellular Biol. 4: 2802-2810 7. Holmberg, C. I., et al (1997) J. Biol. Chem. 272: 6792-6798. 8. Kondo, T., et al. (2000) J. Biol. Chem. 275: 8872-8879. 9. Gusarova, V., et al (2001) J Biol Chem 276(27): 24891-24900. 10. Cwiklinska, H., et al (2003) Int Immunol 15(2): 241-249. 11. Hayem, G., et al (1999) Ann Rheum Dis 58(5): 291-296 12. Ricart, J., et al (1997) Biochem J 324(2): 635-643. 13. White, R.J., et al (2002) Dig Dis Sci 47(8): 1873-1885. 14. de Carcer, G.; et al (2001) EMBO J 20(11): 2878-2884

Additional Info

Recognizes purified bovine and human (alpha & beta) Hsp90 and will detect an ~90kD protein, corresponding to the apparent molecular mass of Hsp90 on SDS-PAGE immunoblots. Immunoprecipitates free and complexed Hsp90. Picks up both hsp90 alpha and beta equally well (detects 100ng of each). Highly recommended over other Hsp90 antibodies for use with samples of fish origin. Species Crossreactivity: Human, monkey, mouse, rat, hamster, guinea pig, rabbit, canine, bovine, sheep, porcine, chicken, frog, plants (cotton, tomato), Drosophila, salmon, carp, rainbow trout, brook trout, beluga, mussel and scallop.