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Heat Shock Protein 90a (Heat Shock Protein HSP 90-alpha, Heat Shock 86kD, HSP 86, HSP86, Renal Carcinoma Antigen NY-REN-38, HSP90AA1, HSP90A, HSPC1, HSPCA)

Cat no: H1832-81A

Heat Shock Protein 90a (Heat Shock Protein HSP 90-alpha, Heat Shock 86kD, HSP 86, HSP86, Renal Carcinoma Antigen NY-REN-38, HSP90AA1, HSP90A, HSPC1, HSPCA)

Cells respond to heat and chemical stress by inducing the synthesis of a group of proteins referred to as heat shock proteins. HSP90 is present in cells at high levels in the cytoplasm and is composed of two separate gene products, HSP90a and HSP90b.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 5-10ug/ml for 2hrs at RT\nImmunoprecipitation: 10ug/mg protein lysate\nImmunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT\nStaining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMAD109 cells, breast carcinoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1832-81A

Size

50ug

Applications

IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes mouse Heat Shock Protein 90a. Species Crossreactivity: human and rat.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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