Heat Shock Protein 90a (HSP90 alpha, HSP86)

Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP 90 is a 90kD protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP 90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, Ah receptor, and some kinases. Studies have shown that murine HSP 90 exists as two forms, HSP 84 and HSP 86, coded by related but separate genes, with 86% homologous amino acid sequences. These forms are analogous to the two forms of human HSP 90, HSP 89 beta and HSP 89 alpha. In an unstressed mouse fibroblast, the basal level of HSP 84 is found to be double that of HSP 86. However, after heat shock, HSP 86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP 86, but not HSP 84, decreases. HSP 84 and HSP 86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1832-82D

Size

100ug

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

PBS, 1 mg/ml BSA, 0.05% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

This product has been used in:Perdew GH et al. Localization and characterization of the 86- and 84-kDa heat shock proteins in Hepa 1c1c7 cells. Exp Cell Res 209:350-6 (1993). <PUBMED:8262153> Chen HS & Perdew GH Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex. J Biol Chem 269:27554-8 (1994). <PUBMED:7961671>Moore SK et al. Characterization of the mouse 84-kD heat shock protein gene family. DNA Cell Biol 9:387-400 (1990). <PUBMED:1976316>Moore SK et al. Murine 86- and 84-kDa heat shock proteins, cDNA sequences, chromosome assignments, and evolutionary origins. J Biol Chem 264:5343-51 (1989). <PUBMED:2925609>

Additional Info

Detects Heat Shock Protein 86 (HSP 86). This antibody does not detect HSP 84. Species Crossreactivity: Crossreacts with Human and Rat. Not yet tested in other species.