It is known that H. pylori strains exhibit a significant degree of diversity. The great variability in the H. pylori genome may explain why not all infected individuals suffer from ulcer. Some H. pylori strains contain particular pathogenic genes such as cytokine associated gene A (CagA), while others lack these genes. The CagA protein of H. pylori has been found to be associated with more severe clinical manifestations, such as ulcer disease and gastric cancer. Thus, discrimination between potentially virulent strains may be relevant
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Matched Pair:
Capture: H1840-45C
Detection: H1840-45F
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
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