HELLS (Lymphoid-specific Helicase, Proliferation-associated SNF2-like Protein, SWI/SNF2-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 6, PASG, SMARCA6, Nbla10143)

A member of the SNF2 family of chromatin remodeling proteins, Lymphoid-specific helicase (HELLS, also known as Lsh) assumes various roles in normal development, survival, de novo or maintenance DNA methylation, gene silencing, and heterochromatin formation and organization. It is shown to serve as cancer progression marker in conjunction with CEP55 and FOXM1 for the detection of head and neck squamous cell carcinoma. It is also reported that expression of Lsh is crucial for meiotic progression in mouse spermatocytes. Lsh is shown to directly associate with the 5' DMR of the Cdkn1c promoter, and thereby regulate its gene expression. Additionally, Lsh is reported as a regulator of repressive chromatin at retrotransposons, and is demonstrated to mediate the silencing of stem cell-specific genes such as Oct4.\n\nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry: 2ug/ml\nWestern Blot: 0.1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa nuclear extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

029494

Size

100ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Huang, J., et al. (2004). Nucleic Acids Res. 32(17):5019-5028. 2. Fan, T., et al. (2005). Development. 132(4): 635-644. 3. Zhu, H., et al. (2006). EMBO J. 25(2):335-345. 4. Waseem, A., et al. (2010). Oral Oncol. 46(7): 536-542. 5. Zeng, W., et al. (2011). Biol Reprod. 84(6): 1235-1241. 6. Xi, S., et al. (2009). Stem Cells. 27(11):2691-2702.

Additional Info

Recognizes human HELLS. Species Crossreactivity: Mouse and rat.