Heme Oxygenase 1 (HO-1, HSP32, Heat Shock Protein 32)

Heme Oxygenase 1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot (ECL): 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 10ug/ml\nOptimal dilutions to be determined by researcher.\n\nPositive Control: \nH1847-66: Heme Oxygenase 1, Recombinant, Human \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1847-69B

Size

50ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can, NHP, Rabbit

Form

Supplied as a liquid in PBS, 0.09% azide, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. G. Vercellotti, et al. Am. J. Physiol. Heart Circ. Physiol. 297, H1243 (2009). 2. H. Christou, et al. J. Cell. Biochem. 105, 1298 (2008). 3. L.R. Barrows, et al. Chem. Res. Toxicol. 16, 113 (2003). 4. F. Altruda, et al. Blood 100, 4201 (2002). 5. M.P. Soares, et al. ; J. Biol. Chem. 277, 17950 (2002). 6. C.H. Contag, et al. J. Mol. Med. 80, 655 (2002).

Additional Info

Recognizes human Heme Oxygenase 1 at ~32kD. Species Crossreactivity: mouse, rat, canine, monkey, rabbit and hamster. Does not detect Heme Oxygenase 2.