Heme Oxygenase 1, Recombinant, Rat (HO-1, HSP32, Heat Shock Protein 32)

Supplier:

United States Biological

Cat no: H1847-67

Heme Oxygenase 1, Recombinant, Rat (HO-1, HSP32, Heat Shock Protein 32)

Heme oxygenase is the rate-limiting microsomal enzyme in the heme degradative pathway. Heme oxygenase catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to form equimolar biliverdin, carbon monoxide, a putative neurotransmitter, and iron. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator; biliverdin and its product bilirubin are potent antioxidants; "free' iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'-UTRs of the mRNAs. To date, 3 forms of heme oxygenases (HO1-3) have been identified. HO-1 or Hsp-32 (EC 1.14.99.3; mouse/rat 289 aa; human 288 aa, chromosome 22; ~88% homology between the species) is an inducible enzyme. Ho-1 is expressed in most tissues with highest levels in spleen. HO-1 gene expression is inducible by heme, suggesting an important role of HO-1 in heme metabolism. Many other agents or conditions related to oxidant damage such as longer wavelength UV radiation, hyperoxia, hypoxia, hydrogen peroxide, glutathione depletion, endotoxin, and, more recently, nitric oxide (NO) have also been found to stimulate HO-1 expression. HO-1 expression has been shown to increase in benign prostatic hyperplasia (BPH) and malignant prostate tissue.\n\nApplications:\nSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions:\nWestern Blot (Colormetric): 50ng\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1847-67

Size

50ug

Applications

WB

Form

Supplied as a liquid in Dulbecco

Purity

(same/more than)90% (SDS-PAGE)

References

1. Elbirt, K.K. and Bonkovsky, H.L. (1999) Proc. Assoc. Am Physicians 111: 438

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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