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Heme Oxygenase 3 (HO-3)

Cat no: H1847-96

Heme Oxygenase 3 (HO-3)

Heme oxygenase is the rate-limiting microsomal enzyme in the heme degradative pathway. Heme oxygenase catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to form equimolar biliverdin, carbon monoxide, a putative neurotransmitter and iron. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator; biliverdin and its product bilirubin are potent antioxidants; "free' iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'-UTRs of the mRNAs. To date, 3 forms of heme oxygenases (HO1-3) have been identified. A third enzyme, HO-3 (rat 290aa ~ 33kD) has been cloned and characterized from rat brain. The HO-3 mRNA is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. The predicted amino acid structure of HO-3 differs from both HO-1 and HO-2 but is closely related to HO-2 (~ 90% similarity). HO-3 sequence has two HRM known to be involved in heme binding. It is a poor heme catalyst. Therefore, HO-3 has a potential regulatory role in heme-dependent cellular processes.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well.\nWestern Blot: 1-10ug/ml using ECL. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide:\nH1847-97: Heme Oxygenase 3, Rat (HO-3) Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1847-96

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Mccourbey WK et al (1997) Eur. J. Biochem. 247, 725-732.

Additional Info

Recognizes rat Heme Oxygenase 3 (HO-3). The rat sequence is not well conserved in HO-1 or HO-2.

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