Hemin BioAssay(TM) Kit

Supplier:

United States Biological

Cat no: H1849

Hemin BioAssay(TM) Kit

Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. It can be detected in various body fluids such as saliva, urine and csf under various pathological states. Free hemin exists in cells at a very minute concentrations (<1uM ? 650ng/ml) exerting regulatory functions such as repression of nonspecific delta-aminolevulinate\nsynthase expression and induction of microsomal hemin oxygenase-1. Hemin can stimulate growth of oral bacteria involved in gingivitis and is an indicator of possible pathological conditions when found in the urine or feces. The Hemin Assay Kit utilizes peroxidase activity in the presence of hemin to provide a simple, exquisitely sensitive assay which causes the conversion of a colorless probe to a strongly colored (lambda = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160pg (10-250fmol) range.\n\nKit Components:\nHemin Assay Buffer 1x25ml\nHemin Probe 1x200ul\nEnzyme Mix 1x1vial\nHemin Substrate 1x1ml\nHemin Standard (1nmol) lyophilized,1x1vial\n\nStorage and Stability:\nStore the kit at -20 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1849

Size

100Tests

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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