Hemoglobin Powder, Bovine (Powder)

Hemoglobin powder is freeze-dried hemoglobin for use in preparing microbiological culture media. Hemoglobin Powder is obtained from desiccated beef blood. Hemoglobin Powder is used with GC Agar in the preparation of Chocolate Agar, Thayer-Martin Medium, Modified Thayer-Martin Medium, Martin-Lewis Agar, and Transgrow Agar. Supplemented with Hemoglobin Powder and growth enrichment, these enriched media are used for the isolation and cultivation of fastidious microorganisms, especially Nesseria spp. and Haemophilus spp. Hemoglobin Powder provides hemin (X factor) required for growth of Haemophilus and for enhanced growth of Neisseria spp. In the preparation of Chocolate Agar, Hemoglobin powder is used in a 2% solution.\n\nHemoglobin is composed of one globin plus four hemes; heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520; however, ultracentrifugal and osmotic pressure\nmeasurements indicate an actual molecular weight four times the minimum molecular weight.\n\nApplication: Used to prepare chocolate agar, Thayer-Martin Medium, Modified Thayer-Martin Medium, Martin-Lewis Agar, and Transgrow Agar.\n\nGrowth:\nNeisseira meningitidis ATCC 13090: Good growth\nHaemophilus influenzae ATCC 10211: Good growth\n\nStorage and Stability:\nStore powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degrees C and used within a short period of time. \n\nCountry of Origin: USA

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