Heparan Sulfate Proteoglycan, Domain 4 (Perlecan, HSPG)

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450kD and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability charateristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling (2).\n\nApplications: \nSuitable for use in Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunocytochemistry: 10ug/ml on primary cultures of hippocampal neurons, fixed with 4% paraformaldehyde in phosphate buffer containing 4% sucrose. The neurons were subsequently permeabilized with 0.25% Triton X-100 for 5 minutes at RT. The fixed and permeabilized cells were blocked with normal donkey serum in Tris buffered saline (TBS) containing 10% Tween. The primary antibody was detected using a FITC-conjugated goat anti-rat secondary antibody.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nMouse-rat hybridoma A7L6 (X63.Ag.8 653 mouse myeloma x Fisher rat splenocytes). Propagated in cell culture.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1890-91

Size

100ug

Applications

ICC

Hosts

Rat

Reactivities

Hum, Mouse, Bov

Form

Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15 sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G chromatography

Isotype

IgG2a,k

References

1. Folkvord et. al., J Histochem Cytochem, 1989; 37:105-113. 2. Couchman et. al., Matrix, 1989; 9:311-321. 3. Horiguchi et. al., J Histochem Cytochem, 1989; 37:961-970. 4. Ljubimov et. al., Int J Cancer, 1992; 50:562-566. 5. Guelstein et. al., Int J Cancer, 1993; 53:269-277. 6. Ljubimov et. al., Lab Invest, 1995; 72:461-473.

Additional Info

Recognizes mouse low density basement membrane heparan sulfate proteoglycan (perlecan) core protein. Does not crossreact with other basement membrane proteins or fibronectin. \nSpecies Crossreactivity: Human and bovine.