Heparan Sulfate Proteoglycan, Domain 4 (Perlecan, HSPG)

Perlecan is a major heparan sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth facors, perlecan hs been stronglyimplicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor. Suppression of perlican causes substantial inhibition of neoplastic growth and neovasacularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogaenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.\n\nApplications: \nSuitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen and acid-alcohol fixed, paraffin embedded sections): 1-2ug/ml for 30 minutes at RT. Staining of acid-alcohol-fixed tissues requires antigen unmasking with 15,000u/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37 degrees C\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTonsil\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1890-92

Size

500ul

Applications

IF, IHC

Hosts

Rat

Reactivities

Hum, Mouse, Bov, Prc

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See H1890-92X.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a,k

References

11. Folkvord et. al., J Histochem Cytochem, 1989; 37:105-113. 2. Couchman et. al., Matrix, 1989; 9:311-321. 3. Horiguchi et. al., J Histochem Cytochem, 1989; 37:961-970. 4. Ljubimov et. al., Int J Cancer, 1992; 50:562-566. 5. Guelstein et. al., Int J Cancer, 1993; 53:269-277. 6. Ljubimov et. al., Lab Invest, 1995; 72:461-473.

Additional Info

Recognizes mouse Heparan Sulfate Proteoglycan. Does not crossreact with laminin, fibronectin or dermatran sulfate proteoglycan. Species Crossreactivity: human, bovine and porcine