Heparan Sulfate Proteoglycan (Perlecan, HSPG)

Human perlecan (HSPG2) core protein is a large, complex, multidomain heparan sulfate proteoglycan with a MW of ~467kD (determined from the cDNA sequence). Post-translational modifications of HSPG2 can increase the MW to ~850kD. Perlecan is comprised of five domains. Domain I (N terminal) is predicted to be the site of heparan sulfate linkage to 3 glycosaminoglycans. Domain II is homologous to the LDL receptor. Domain III shares homology with the short arm of laminin A and B chains. Domain IV contains IgG-like repeats similar to those found in N-CAM and has a hydrophobic region suitable for interacting with the membrane. Domain V has regions similar to EGF and the G-domain of laminin A chain. In skin, perlecan is synthesized exclusively by the connective tissue in the dermal layer. Perlecan has been detected in many human tissues, including; breast, colon, liver, lymph nodes, pancreas, and prostate, among others. Proteins similar to perlecan have been identified in colon carcinoma, EHS tumor, and fibrosarcoma cells. \n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry (Frozen or FFPE): 5-10ug/ml. Note: Formalin-fixed, paraffin sections require pretreatment with 0.1% protease for 30 minutes at RT prior to the blocking step.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHuman breast, colon, liver, lymph nodes, pancreas and prostate tissues.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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