Heparan Sulfate Proteoglycan (Perlecan, HSPG)

Heparan sulfate proteoglycans (HSPGs) are found on cell surfaces and in the extracelluar matrix of all mammalian tissues, particularly in the core of the basement membrane. HSPGs found in the brain are dystroglycan, N-syndecan, glypican, and perlecan. The composition of HSPGs differs in the protein core, and is thought to determine the location of HSPG in the cell membrane (syndecan and glypican) and extracellular matrix (perlecan and dystroglycan). The postulated functions of HSPG include cell proliferation, differentiation, adhesion, migration and morphogenesis.\n\nHeparan Sulfate Proteoglycan antibodies, in tissues, react strongly and uniformly with basement membranes. The reactivity is independent of the galactosaminoglycan moieties; therefore, the epitope is not sensitive to heparinase treatment.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. \n\nRecommended Dilution:\nImmunohistochemistry: 1:100; Detected HSPG in large cell carcinoma tissue.\nImmunocytochemistry: 1:500\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa and A431 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1890-93C

Size

100ug

Applications

ICC, IHC, IP, WB

Hosts

Rat

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a,k

References

1. Hagen, S., et al., J. Biol. Chem. 286(10): 7261-7269 (1993). 2. Brown, C.T., et al., J. Biol. Chem. 274(11): 7111-7119 (1999). 3. Couchman, J., et al., Matrix 9: 311-321 (1989). 4. Horiguchi, Y., et al., J. Histochem. and Cytochem. 37(7): 961-970 (1989). 5. Ljubimov, A.V., et al., Lab. Invest. 72(4): 461-473 (1995).

Additional Info

Recognizes human Heparan Sulfate Proteoglycan (Perlecan). Recognizes domain IV of the core protein of the large Heparan Sulfate Proteoglycan.