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Heparin Cofactor II, Human (HCII)

Cat no: H1892-75

Heparin Cofactor II, Human (HCII)

Inhibition of Thrombin by Heparin Cofactor II: Thrombin (IIa), heparin (HEP) and heparin cofactor II (HCII) interact to form a ternary complex via a random order bireactant mechanism. Heparin subsequently dissociates from the complex as thrombin forms a covalent complex with the heparin cofactor II.\n

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SPECIFICATIONS

Catalog Number

H1892-75

Size

100ug

Form

Supplied as a liquid in 50% glycerol/H2O.

Purity

Heparin cofactor II is prepared from fresh frozen plasma by a modification of the procedure of Griffith, et al., (9). Purity is (same/more than)95% by SDS PAGE and activity is determined based on thrombin inhibition in the presence of dermatan sulfate.

References

1. Bringinshaw, G.W., Shanberge, J.M., Arch. Biochem. Biophys. 161: 683 (1974). 2. Tollefsen, D.M., et al., J. Biol. Chem. 257: 2162 (1982). 3. Pratt, C.W., et al., Ann. N.Y. Acad. Sci. 556: 104 (1989). 4. Ragg, H., Nucleic Acids Res. 14: 1073 (1986). 5. Church, F.C., et al., Arch. Biochem. Biophys. 259: 331 (1987). 6. Hortin, G., et al., J. Biol. Chem. 261: 15,827 (1986). 7. Church, F.C., et al., PNAS USA 82: 6431 (1985). 8. Tollefsen, D.M., Blank, M.K., J. Clin. Invest. 68: 589 (1981). 9. Griffith, M.J., et al., J. Biol. Chem. 260: 2218 (1985). 10. Tollefsen, D.M., et al., J. Biol. Chem. 258: 6713 (1983). 11. Griffith, M.J., Marbet, G.A., Biochem. Biophys. Res. Comm. 112: 663 (1983). 12. Abildgaard, U., Larsen, M.L., Thromb. Res. 35: 257 (1984). 13. Griffith, M.J., et al., Biochemistry 24: 6777 (1985). 14. Parker, K., Tollefsen, D.M., J. Biol. Chem. 260: 3501 (1985).

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