Hepatitis B Surface Antigen, adw, Recombinant (HBsAg)

The hepatitis B virus is an important cause of acute and chronic liver disease. It is also the most common form of parenterally transmitted viral hepatitis. The virus is estimated to affect some 350 million people worldwide. Immunization against hepatitis B is an important component of preventative medicine and is offered to all children in approximately 30 countries.\n\nHepatitis B virus (HBV) belongs to the Hepadnaviridae family and infection with HBV is usually more serious than infection with hepatitis A virus. In some individuals the infection is not cleared and followed by persistent intracellular viral carriage that can lead to the development of hepatocelular carcinomas. There are currently 400 million HBV carriers worldwide making HBV one of the most common human pathogens.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of HBsAg.\n\nBiological Activity:\nWas tested in ELISA with anti-HBsAg antibodies\n\nApplications:\n1. Immunochromatography (capture and conjugate). 2. Immunogen for monoclonal antibody production. 3. ELISA\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H1909-18A

Size

100ug

Applications

ELISA

Form

Supplied as a liquid in 0.05M phosphate buffer, pH 7.2, 0.2M sodium chloride.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. [The content of immunoglobulins in blood serum of asymptomatic carriers of HBsAg with alcoholism] Klin Lab Diagn 2004 Nov;(11):13-6 2. [Effects of oxymatrine on serum levels of Th1/Th2 type cytokines in HBsAg transgenic mice.] Dong YH, Xi HL, Kang AJ, Wang QH, Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2004 Sep;18(3):277-80 3. Pre-vaccination screening for hepatitis B infection in high-risk population: Is HBsAg alone adequate? Nijhawan S, Joshi A, Shende A, Indian J Gastroenterol 2004 Nov-Dec;23(6):229-30 4. [HBsAg autologous red cell agglutination assay] Gong H, Wang A, Xu L, Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 1997 Mar;11(1):78-80 5. [Immune responses to pcDNA3.1 hisB-IL-15 and HBsAg DNA vaccine in BALB/c mice.] Yang CY, Su XS, He Y, Zhonghua Gan Zang Bing Za Zhi 2004 Dec 20;12(12):758-759 6. Liver histology in patients with HBsAg negative anti-HBc and anti-HCV positive chronic hepatitis. Sagnelli E, Pasquale G, Marrocco C, Scolastico C, J Med Virol 2005 Feb;75(2):222-6.