Hepatitis C Virus Core, Nucleocapsid, Recombinant (HCV) (HRP)

The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However, the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways In addition,HCV core antigen is a recently developed marker of hepatitis C infection. It is remarkably efficient at establishing persistent infection, suggesting that it has evolved one or more strategies aimed at evading the host immune response. T cell responses, including interferon-gamma production, are severely suppressed in chronic HCV patients. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.\n\nThe protein contains the HCV core nucleocapsid immunodominant regions.\n\nSpecificity:\nImmunoreactive with sera of HCV-infected individuals\n\nApplications:\nRecombinant HCV Core Antigen may be used in ELISA and Western Blot, excellent for detection of HCV with minimal specificity problems.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1920-17S

Size

100ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 1mM EDTA, 1.5M urea, 50% glycerol. Conjugated to HRP.

Purity

(same/more than) 95% by 10% PAGE. Chromatographically purified.\n

References

1. Hepatitis C virus core, NS3, NS5A, NS5B proteins induce apoptosis in mature dendritic cells. Siavoshian S, Abraham JD, Paule Kieny M, J Med Virol 2005 Jan 12;75(3):402-411 2. Benefit of hepatitis C virus core antigen assay in prediction of therapeutic response to interferon and ribavirin combination therapy. Takahashi M, Saito H, Atsukawa K, J Clin Microbiol 2005 Jan;43(1):186-91 3. Hepatitis C virus core protein, cytochrome P450 2E1, and alcohol produce combined mitochondrial injury and cytotoxicity in hepatoma cells. Otani K, Korenaga M, Li K, Wang T, Gastroenterology 2005 Jan;128(1):96-107 4. Molecular determinants for subcellular localization of hepatitis C virus core protein. Suzuki R, Sakamoto S, Rikimaru A, Iwasaki T, J Virol 2005 Jan;79(2):1271-81 5. Hepatitis C virus core protein is efficiently released into the culture medium in insect cells. Choi SH, Kim SY, Kim YJ, J Biochem Mol Biol 2004 Nov 30;37(6):735-40 6. Type 1 interferon augments DNA-based vaccination against hepatitis C virus core protein. Gehring S, Gregory SH, Wands JR, J Med Virol 2005 Feb;75(2):249-57.