Hepatitis C Virus NS3, Recombinant (HCV) (HRP)

The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However, the mechanisms of core-mediated transcriptional regulation remain poorly understood.Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways In addition, HCV core antigen is a recently developed marker of hepatitis C infection. It is remarkably efficient at establishing persistent infection, suggesting that it has evolved one or more strategies aimed at evading the host immune response. T cell responses, including interferon-gamma production, are severely suppressed in chronic HCV patients. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.\n\nThe protein contains the HCV nonstructural NS3 protein immunodominant regions.\n\nSpecificity:\nImmunoreactive with sera of HCV-infected individuals\n\nApplications:\nRecombinant HCV NS3 Antigen may be used in ELISA and Western Blot, excellent for detection of SARS with minimal specificity problems.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1920-22A6

Size

100ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 1mM EDTA, 1.5M urea, 50% glycerol. Conjugated to HRP.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Antiviral compounds from traditional Chinese medicines Galla Chinese as inhibitors of HCV NS3 protease. Duan D, Li Z, Zhang W, Bioorg Med Chem Lett 2004 Dec 20;14(24):6041-4 2. Synthesis of BILN 2061, an HCV NS3 protease inhibitor with proven antiviral effect in humans. Faucher AM, Bailey MD, Brochu C, Gorys V, Org Lett 2004 Aug 19;6(17):2901-4 3. In vitro selection of RNA aptamers against the HCV NS3 helicase domain. Nishikawa F, Funaji K, Nishikawa S, Oligonucleotides 2004;14(2):114-29 4. P4 cap modified tetrapeptidyl alpha-ketoamides as potent HCV NS3 protease inhibitors. Sun DX, Liu L, Kolykhalov A, Yip Y, Bioorg Med Chem Lett 2004 Aug 16;14(16):4333-8 5. Capped dipeptide phenethylamide inhibitors of the HCV NS3 protease. Nizi E, Koch U, Marchetti A, Gardelli C, Bioorg Med Chem Lett 2004 May 3;14(9):2151-4 6. Double-stranded DNA-induced localized unfolding of HCV NS3 helicase subdomain 2. Liu D, Windsor WT, Protein Sci 2003 Dec;12(12):2757-67.