Hepatitis C Virus NS5, Genotype 2, Recombinant (HCV)

The hepatitis C virus (HCV) NS5A gene product is a phosphorylated 56-58kD nonstructural protein that displays a multitude of activities related to enhancement of viral pathogenesis. Although associated with other viral encoded proteins as part of the viral replicase complex positioned on the cytoplasmic side of the endoplasmic reticulum, a role for NS5A in viral replication has not been defined.Truncated versions of NS5A can act as transcriptional activators, while other recently characterized interactions of NS5A with cellular proteins indicate its pleiotropic role in HCV-host interactions.\n\nIt has been shown that NS5B is a membrane-associated protein, which contains a C-terminal domain comprising 21 hydrophobic amino acids that is responsible for membrane anchorage. NS5B may form a complex with cellular proteins or other HCV nonstructural proteins, including NS3, the viral protease and helicase; NS4A, a cofactor of NS3 protease activity; and NS5A, a phosphoprotein containing a putative interferon sensitivity region. Although the HCV replication mechanism is not clearly understood, the essential role of NS5B polymerase in the HCV replication and infection process has been demonstrated in chimpanzees. Accordingly, it has been viewed as an attractive target for antiviral intervention.\n\nThe protein contains the HCV NS5 Genotype 1 immunodominant regions.\n\nApplications: \nRecombinant HCV NS5 Antigen may be used in ELISA and Western blots, excellent for detection of HCV with minimal specificity problems. Immunoreactive with sera of HCV-infected individuals.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1920-28D

Size

100ug

Form

Supplied as a liquid in 50mM Tris, pH 8.0 and 5mM EDTA.

Purity

Purified by proprietary chromatographic techniques. (same/more than) 95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

References

1. Hepatitis C virus (HCV) NS5A protein downregulates HCV IRES-dependent translation. J Gen Virol 2005 Apr;86(Pt 4):1015-25.\n2. Inhibitors of HCV NS5B polymerase: synthesis and structure-activity relationships of N-1-heteroalkyl-4-hydroxyquinolon-3-yl-benzothiadiazines. Bioorg Med Chem Lett 2005 Mar 15;15(6):1577-82.\n3. Characterization of a HCV NS5A protein derived from a patient with hepatoma. Biochem Biophys Res Commun 2005 Feb 11;327(2):516-22.\n4. Inhibitors of the HCV NS5B polymerase: new hope for the treatment of hepatitis C infections. Curr Opin Investig Drugs 2004 Aug;5(8):838-50.\n5. Discovery of pyrano[3,4-b]indoles as potent and selective HCV NS5B polymerase inhibitors. J Med Chem 2004 Dec 16;47(26):6603-8.\n6. HCV NS5b RNA-dependent RNA polymerase inhibitors: from alpha,gamma-diketoacids to 4,5-dihydroxypyrimidine- or 3-methyl-5-hydroxypyrimidinonecarboxylic acids. Design and synthesis. J Med Chem 2004 Oct 21;47(22):5336-9.