Hepatitis Delta Virus, Mosaic, Recombinant (HDV)

Hepatitis delta virus (HDV) contains a circular RNA which encodes a single protein, hepatitis delta antigen (HDAg). HDAg exists in two forms, a small form (S-HDAg) and a large form (L-HDAg). S-HDAg can transactivate HDV RNA replication. Post-translational modifications, such as phosphorylation and acetylation, of S-HDAg can modulate HDV RNA replication. The RNAs undergo different forms of posttranscriptional RNA processing. Transcripts of both the genomic RNA and its exact complement, the antigenomic RNA, undergo ribozyme cleavage and RNA ligation. In addition, antigenomic RNA transcripts can undergo 5' capping, 3' polyadenylation, and even RNA editing by an adenosine deaminase.\n\nThe protein contains the HDV immunodominant regions,amino acids: (1-108)(151-209).\n\nSpecificity:\nImmunoreactive with sera of HDV-infected individuals\n\nApplications:\nSuitable for use in ELISA and Western Blot. Excellent for detection of HDV with minimal specificity problems. Other applications not determined.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1926-45

Size

100ug

Form

Supplied as a liquid in 50mM Tris, pH 8, 8mM Urea.

Purity

(same/more than)95% by 10% SDS-PAGE (coomassie staining). Purified by proprietary chromatographic technique.

References

1. Hepatitis delta virus antigen is methylated at arginine residues, and methylation regulates subcellular localization and RNA replication. Li YJ, Stallcup MR, J Virol 2004 Dec;78(23):13325-34 2. [Hepatitis delta virus (HDV) ribozymes] Legiewicz M, Ciesiolka J, Postepy Biochem 2004;50(1):19-31 3. [Replication of hepatitis delta virus] Suzuki T, Nippon Rinsho 2004 Aug;62 Suppl 8:393-7 4. [Hepatitis delta virus (HDV) virions, HDV-RNA and HDV related proteins] Goto T, Kato N, Nippon Rinsho 2004 Aug;62 Suppl 8:389-92 5. Identification of novel HLA-A*0201-restricted CD8+ T-cell epitopes on hepatitis delta virus. Huang YH, Tao MH, Syu WJ, J Gen Virol 2004 Oct;85(Pt 10):3089-98 6. Terbium-mediated footprinting probes a catalytic conformational switch in the antigenomic hepatitis delta virus ribozyme. Harris DA, Tinsley RA, J Mol Biol 2004 Aug 6;341(2):389-403.